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G.W. Warr

Publications and source records attributed to G.W. Warr.

5 recordsLinked to original sources
USGS2006

A lymphosarcoma of thymic origin in the rainbow trout, Salmo gairdneri Richardson

A rainbow trout with a malignant lymphoma of probable thymic origin showed direct spread of the tumour to the gills, metastases to the liver and head kidney, and the presence of abnormal circulating lymphocytes. The cells of the tumour possessed plasma membrane immunoglobulin detectable by immuno–fluorescence. Examination of the tumour cells by transmission electron microscopy revealed no evidence of virus associated with the cells. Infectious viruses could not be detected in the tumour tissue, and the tumour was not transmissible by injection of live tumour cells into young rainbow trout. Attempts to establish the tumour in long–term culture were unsuccessful.

Journal of Fish Diseases↗
USGS1983

Thymocyte plasma membrane of the rainbow trout, Salmo gairdneri : Associated immunoglobulin and heteroantigens

1. Thymic lymphocytes of the rainbow trout, S. gairdneri were disrupted and a plasma membrane containing fraction isolated by differential and buoyant density centrifugation. 2. Radioiodine introduced into the membrane by the lactoperoxidase catalyzed reaction and immunoglobulin (identified by radioimmunoassay with monoclonal antibody) both copurified in the plasma membrane fraction. 3. Rabbit antibody raised to the plasma membrane fraction showed a strong reaction with trout lymphocytes in immunofluorescence, was mitogenic for trout lymphocytes, and recognized lymphocyte membrane heteroantigens of molecular weight > 70,000 in the thymus and 45,000–95,000 in the head kidney.

Comparative Biochemistry and Physiology, Part B: C↗
USGS1979

Membrane immunoglobulin is present on thymic and splenic lymphocytes of the trout Salmo gairdneri

Three rabbit antisera raised to trout IgM showed positive immunofluorescent reactions with lymphocytes of trout spleen and thymus. These antisera showed no detectable cross-reactivity with keyhole limpet hemocyanin, as determined by direct radioimmune precipitation and adsorption, and did not appeare to react significantly with carbohydrate moieties on trout IgM, as determined by the failure of proteolytic digests of trout IgM to inhibit in radioimmunoassay against intact trout IgM. Membrane immunoglobulin determinants were detectable on the membrane of both thymocytes and splenocytes with the lactoperoxidase-catalyzed iodination reaction. Radioimmunoassay of lysates of lymphocytes confirmed the association of immunoglobulin with trout thymocytes. By radioimmunoassay, lysates of thymic and splenic lymphocytes contained similar amounts of immunoglobulin, equivalent to approximately 8 × 10 4 molecules of IgM per cell (calculated as the monomer µ 2 L 2 ).

Journal of Immunology↗