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Donald F. Amend

Publications and source records attributed to Donald F. Amend.

At least 19 recordsLinked to original sources
USGS2006

Some aspects of pathogenesis of infectious hematopoietic necrosis (IHN)

The histopathogenesis of infectious haematopoietic necrosis (IHN) virus infection was studied by exposing juvenile sockeye salmon ( Oncorhynchus nerka ) to the IHN virus. Fish samples were taken every 24 h for histological examination and for determination of virus concentration. A close correlation was found between histopathological changes and virus concentration. The most significant changes occurred 4 days after exposure. The haematopocitic tissue of the kidney was the most extensively involved but minor degenerative changes were seen in the liver, pancreas, and in the granular cells of the digestive tract. On the 4th day, maximum tissue concentration of virus was reached and the mortality increased. By the 5th day, 90% of the samples showed extensive pathological changes in the kidney, together with variable changes in spleen, liver, pancreas, and gut. Similarities in the histopathogenesis of IHN, Oregon sockeye disease (OSD), Sacramento River chinook disease (SRCD) and viral haemorrhagic septicaemia (VHS), are discussed.

Journal of Fish Biology↗
USGS2006

Efficacy of certain disinfectants against infectious pancreatic necrosis virus

The virucidal properties of iodophor, chlorine (sodium hypochlorite), formalin, thimerosal (organic mercurial compound), malachite green, and acriflavine were tested on infectious pancreatic necrosis virus (IPNV). Iodine and chlorine showed good activity, but efficacy depended on the concentration of virus, the presence of organic matter (calf serum), and water p H. Water hardness (0-300 mg 1 −1 as CaCO 3 ) did not affect virucidal activity. In a 5 min exposure, 4 mg 1 −1 available iodine inactivated 10 3.9 TCID 50 m1 −1 IPNV but 16 mg 1 −1 iodine were needed for inactivation of 10 6.3 TCID 50 m1 −1 . The addition of 0-5% calf serum significantly reduced the iodine concentration and the virucidal activity. In comparison, 4 mg 1 −1 chlorine were needed to inactivate 10 46 TCID 50 m1 −1 IPNV in 5 min. However, the addition of 0-07 % serum greatly reduced the chlorine concentration and extended the virucidal contact time to 30 min or more. IPNV at 10 6.3 TCID 60 m1 −1 was not inactivated by exposures for 60 min to 0-2% formalin, 10 min to 0-2% thimerosal, 60 min to 5 mg 1 −1 malachite green, or 20 min to 500 mg 1 −1 acriflavine. However, acriflavine at 0-5 mg 1 −1 in cell culture media prevented the development of cytopathology caused by IPNV and may be useful in the treatment of the disease.

Journal of Fish Biology↗
USGS2004

The controversy about salmon hatcheries

The use of hatcheries has been a subject of lengthy debate in the management of salmon and trout resources in the Pacific Northwest. The problem has resulted in part from the wide distribution of hatchery fish in circumstances where natural populations were disadvantaged by management policy involving hatchery fish and the confusion of the effects of management with the effects of artificial propagation. Recently, the controversy has been epitomized by the recommendations to fisheries management agencies that excess hatchery fish should not be allowed to spawn in the wild, and hatchery fish should be excluded from salmon populations listed under the Endangered Species Act. The authors of the present article disagree with those recommendations and conclude that hatchery fish have an important role in recovery and supplementation of wild stocks. The present article is an attempt to help give balance to the discussion by providing a different perspective on hatchery fish and the literature pertaining to artificial propagation.

Fisheries Magazine↗
USGS1977

Immunization of sockeye salmon (Oncorhynchus nerka) against vibriosis using the hyperosmotic infiltration technique

Various procedures of hyperosmotic infiltration (HI) and intraperitoneal injection were used to vaccinate sockeye salmon ( Oncorhynchus nerka ) with killed Vibrio anguillarum . Excellent protection was evident against experimentally induced vibriosis in the groups immunized by HI with 10 × Hanks' balanced salt solution (HBSS), 1 × HBSS with 8.0% NaCl and 5.3% NaCl, as well as in the injected groups. Comparisons were made among the various immunization methods by vaccinating fish with ten-fold serial dilutions of bacterin, then challenging them by the water contact method after 6 or 9 weeks. Protection was somewhat better with 10 × HBSS than with 5.3% NaCl, and 1 × HBSS containing 8.0% NaCl was markedly superior to the vaccination of fish without hyperosmotic treatment. Agglutinin titers did not exceed 1 : 8 in any group.

Aquaculture↗
USGS1977

Immunization of pacific salmon: comparison of intraperitoneal injection and hyperosmotic infiltration of Vibrio anguillarum and Aeromonas salmonicida bacterins

Two methods of immunizing fish, intraperitoneal (i.p.) injection and hyperosmotic infiltration, were compared for control of vibriosis and furunculosis in pen-reared coho salmon ( Oncorhynchus kisutch ) and chinook salmon ( O . tshawytscha ). Both methods provided significant protection against vibriosis under field test conditions. In coho salmon, hyperosmotic infiltration provided the best protection and fastest rise in antibody titer of seven treatments tested. In chinook salmon, hyperosmotic infiltration of Vibrio anguillarum and Aeromonas salmonicida vaccines resulted in 83.3% survival in comparison with 28.7% survival in controls. Both i.p. injection and hyperosmotic infiltration of V . anguillarum and A . salmonicida bacterins resulted in production of serum antibodies specific for each respective pathogen. Vaccination with bivalent V . anguillarum – A . salmonicida vaccines produced antibodies to both pathogens, and provided protection against vibriosis. Growth rates of vaccinated coho salmon were not significantly different from controls.

Journal of the Fisheries Research Board of Canada↗
USGS1976

Prevention and control of viral diseases of salmonids

Three viral diseases of salmonids are of worldwide concern: infectious pancreatic necrosis (IPN) viral hemorrhagic septicemia (VHS), and infectious hematopoietic necrosis (IHN). Six principal approaches are being used to prevent or control these diseases: 1) preventing contact o the pathogen with the host, 2) environmental manipulation, 3) immunization, 4) chemotherapy, 5 selective breeding for disease resistance, and 6) reducing stress conditions which augment disease conditions. Preventing the introduction of a pathogen into a new stock of fish has been accomplished mainly by implementing stringent laws to prevent transport of infected fish into uninfected areas. Stocks of fish already infected are sometimes destroyed, and the hatchery is disinfected and restocked with fish free of specific pathogens. Environmental manipulation (elevated water temperature) has been successfully used to control IHN. Chemotherapeutics such as povidone-iodine for IPN and benzipyrene for IHN show promise of controlling mortalities; however, the practicality of using these drugs to eliminate the carrier fish has not been evaluated. Salmonids are capable of developing immune responses to viruses; however, development of effective vaccines, selective breeding for disease resistance, and identification of stress conditions which augment disease are still in the experimental phase.

Journal of the Fisheries Research Board of Canada↗
USGS1975

Detection and transmission of infectious hematopoietic necrosis virus in rainbow trout

Detection and transmission of Infectious Hematopoietic Necrosis Virus in rainbow trout ( Salmo gairdneri ) was studied at a commercial trout hatchery. Transmission of virus was demonstrated via water, feed and contaminated eggs. If eggs from carrier females were incubated several weeks in virus-free water, the resulting fry did not become infected. However, if fry subsequently became infected they were lifetime carriers. Infectious virus was readily detectable in most tissues of moribund fish; in carriers it was detected in sex products of spawning fish, and in samples from the intestine of post-spawning fish, but not in samples from blood, feces, kidney, or liver. The carrier rate was not significantly different between sexes. It was concluded that adult carriers are the reservoir of infection and that transmission occurs primarily when carriers shed virus and expose susceptible fish or eggs.

Journal of Wildlife Diseases↗
USGS1974

Pathophysiology of infectious hematopoietic necrosis virus disease in rainbow trout (Salmo gairdneri): early changes in blood and aspects of the immune Response after Injection of IHN Virus

Juvenile rainbow trout ( Salmo gairdneri ) were injected with infectious hematopoietic necrosis (IHN) virus and various hematological and blood chemical changes were monitored over 9 days. The packed cell volume, hemoglobin, red blood cell count, and plasma bicarbonate were significantly depressed by day 4. Plasma chloride, calcium, phosphorus, total protein, and blood cell types did not change during the 9 days. Furthermore, plasma LDH isozyme was significantly increased by the fourth day, and fish infected with infectious pancreatic necrosis virus, Vibrio anguillarum , Aeromonas salmonicida , and redmouth bacterium did not show specific LDH isozyme alterations. Acid-base alterations occurred at 10 C but not at 18 C. The acid-base imbalance and elevation of the LDH isozyme were consistently associated with the early development of the disease.The immune response after injection of IHN virus was determined and protection from disease was tested by passive immunization. Actively immunized fish developed IHN-neutralizing antibodies within 54 days after injection of virus, and the antibodies were protective when juvenile fish were passively immunized and experimentally challenged with IHN virus.

Journal of the Fisheries Research Board of Canada↗
USGS1974

Comparative toxicity of two Iodophors to rainbow trout eggs

Toxicity of Wescodyne (R) and Betadine (R) to eyed eggs was not adversely affected by water hardness (as calcium and magnesium) or by exposure periods up to 60 min. Both iodophors were much more toxic below pH 6.0 than at pH 8.0. In general Wescodyne was slightly more toxic than Betadine. Significant egg loss occurred if freshly fertilized eggs were water-hardened in either iodophor at 100 ppm of iodine, but egg loss at 25 ppm of iodine or at 100 ppm if the eggs were disinfected 30 min after water hardening was comparable to the control. Also, there was no effect on the egg mortality or fry development following single or multiple exposures after eggs were water hardened. At pH 6.0 and above, Wescodyne and Betadine at 100 ppm iodine in a 15-min dip would be safe to use on rainbow trout eggs at any stage of development after water hardening. Recommendations and precautions for hatchery use are given.

Transactions of the American Fisheries Society↗
USGS1972

Virucidal activity of two Iodophors to salmonid viruses

Wescodyne ® and Betadine ® , organic iodine complexes, were compared in vitro for virucidal activity against infectious hematopoietic necrosis (IHN), infectious pancreatic necrosis (IPN), and viral hemorrhagic septicemia (VHS) viruses. Both iodophors were about equally effective on all three viruses. Each iodophor completely destroyed IHN virus within 30 sec at 12 ppm iodine, and was not affected by water hardness. Virucidal activity, however, was reduced at p H levels above 8.0 and in the presence of organic matter. Wescodyne was also compared with seven disinfectants commonly used in fish hatcheries, for virucidal properties against IHN virus. Wescodyne and chlorine were the only disinfectants to completely destroy the virus. Either Wescodyne or Betadine would effectively destroy the salmonid viruses at less than 25 ppm iodine within 5 min in solutions near neutrality.

Journal of the Fisheries Research Board of Canada↗
USGS1972

Survey for infectious hematopoietic necrosis (IHN) virus in Washington salmon

A virus disease of juvenile sockeye salmon ( Oncorhynchus nerka ) has been a problem in Washington hatcheries since first reported by Rucker [9] in 1953. Presumably, the same disease has occurred in Oregon, and it is now referred to as the Oregon, and it is now referred to as the Oregon sockeye disease (OSD) or the sockeye salmon virus (SSV) [8,12]. The primary source of the disease was thought to be from the feeding of raw sockeye salmon viscera, and the incidence decreased when pasteurized diets were used [5]. However, sporadic attacks continue to occur even though pelleted diets containing pasteurized fish products are fed.

Progressive Fish-Culturist↗
USGS1972

An improved method for isolating viruses from asymptomatic carrier fish

This paper describes a method using elevated levels of penicillin, streptomycin, and nystatin instead of filters to control bacteria and mold contaminants in specimens processed for virus isolation. Filters were shown to significantly reduce the virus concentration. Virus and tissue cultures were not affected by this procedure. In field tests nearly three times more specimens were positive for virus with this method than with the widely used filter technique. Moreover, the cost of materials was less. This method is recommended for inspection and certification purposes.

Transactions of the American Fisheries Society↗
USGS1970

Control of infectious Hematopoietic Necrosis virus disease by elevating the water temperature

Studies were performed to determine if increasing water temperatures could control infectious hematopoietic necrosis virus (IHN) disease in sockeye salmon ( Oncorhynchus nerka ). Mortalities could be prevented if the water temperature was raised to at least 18 C within the first 24 hr after infection of the fish and if the fish were maintained at this temperature for 4–6 days. The disease did not recur after the elevated temperature treatment, but the fish would still contract the disease if they were reinfected. Reasons for the protecting action are discussed.

Journal of the Fisheries Research Board of Canada↗
USGS1970

Morphology of certain viruses of Salmonid Fishes. I. in vitro studies of some viruses causing Hematopoietic Necrosis

An electron microscope study was performed on three virus isolates that caused hematopoietic necrosis in salmonid fishes: infectious hematopoietic necrosis (IHN), Oregon Sockeye Disease (OSD), and Sacramento River Chinook Salmon Disease (SRCD). All three isolates were examined by negative staining of fathead minnow (FHM) monolayer tissue culture concentrates and IHN virus was also examined in thin sections of FHM cells. Viruslike particles were observed in infected tissues, but similar structures were not found in uninfected cultures. All three isolates were bullet-shaped, but oval and truncated forms were also observed. Mean measurements of particles from IHN-virus-infected tissue were 158 × 90 mμ. They consisted of an outer coat 15 mμ thick, a core 60 mμ in diameter, subunits about 5 mμ, and an axial pore about 20 mμ in diameter. These particles also were seen budding from the cytoplasmic membrane. Similar particles from SRCD were 159 × 90 mμ and isolates from OSD were 181 × 91 mμ. The three isolates were morphologically indistinguishable from one another and the greater length of OSD was considered insignificant. IHN, SRCD, and OSD viruses were tentatively placed in the rhabdovirus group, but serological studies are needed to determine if they are antigenically identical or should be included as separate members. Biochemical and physical characteristics of these viruses and a comparison with other salmonid viruses is also discussed.

Journal of the Fisheries Research Board of Canada↗
USGS1970

Morphology of certain viruses of Salmonid fishes. II. In vivo studies of infectious Hematopoietic Necrosis Virus

Juvenile sockeye salmon ( Oncorhynchus nerka ) were injected with the infectious hematopoietic necrosis (IHN) virus, and tissue samples from the anterior kidney, spleen, liver, intestine, and pyloric caeca of moribund fish were prepared for electron microscopy. Bullet-shaped virus particles measuring 158 × 90 mμ were observed in the hematopoietic tissues of the anterior kidney and spleen. Virus particles were also observed in the outer connective tissues of the pancreas or pyloric caeca, or both. No virus was found in the intestine or liver. The healthy appearance of erythrocytes, reticular cells, and endothelial cells in necrotic areas of the spleen and anterior kidney, and the absence of lymphocytes in these areas, suggested that lymphocytes might be one source of the virus.

Journal of the Fisheries Research Board of Canada↗
USGS1970

Retention of mercury by salmon

Consuming fish that have been exposed repeatedly to mercury derivatives is a potential public health hazard because fish can accumulate and retain mercury in their tissues (Rucker, 1968). Concern has been expressed in the United States because mercurials have been used extensively in industry and as prophylactic and therapeutic agents in fish hatcheries. Rucker and Amend (1969) showed that yearling rainbow trout (Salmo gairdneri) and chinook salmon (Oncorhynchus tshawytscha) exposed to mercurials accumulated excessive amounts of mercury in many tissues. Further, Rucker and Amend (1969) concluded that wild fish that ate mercury-contaminated fish also could contain high mercury levels. Although mercury was eliminated from most tissues within several months, substantial levels remained in the kidney for more than 33 weeks after the last exposure. Since high levels of mercury can be retained in the kidney for an undetermined time, it is possible that returning adult salmon exposed to mercurials as juveniles could constitute a potential hazard to public health. The purpose of this study was to determine whether such fish contained high residual levels of mercury.

Progressive Fish-Culturist↗