Geology topics

David M. Walters

Publications and source records attributed to David M. Walters.

At least 19 recordsLinked to original sources

USGS2026

Beyond barriers: Fish assemblage recovery following dam removal on the Cuyahoga River, a Lake Erie tributary

Dam removals are increasing globally, yet ecological outcomes vary widely because biological recovery depends on post-removal connectivity and access to source populations. We evaluated how multiple dam removals and remaining fragmentation influenced fish assemblage recovery in the Cuyahoga River (OH, United States), a historically polluted Great Lakes tributary now undergoing extensive remediation and restoration. Using fish assemblage data collected from 1984 to 2024 across reaches downstream, within, and upstream of the Gorge Plant Dam (GPD), an 18-m barrier slated for removal, we quantified spatial and temporal change with ordination, trajectory, and distance-based redundancy analyses. Downstream assemblages showed a clear shift through time and gained 15 newly detected taxa after the 2020 Brecksville Dam removal increased longitudinal connectivity to Lake Erie by 39 river kilometers, more than doubling free-flowing habitat below GPD. In contrast, assemblages upstream of GPD exhibited limited change and patterns consistent with redistribution of resident species rather than colonization-driven turnover. Across the full time series, site position relative to GPD and the rate of dam removal were the strongest predictors of assemblage variation, with habitat gradients (e.g. depth, velocity, turbidity, and canopy cover) further differentiating reaches. Together, these results show that connectivity and barrier position shape assemblage recovery following dam removal. Most newly detected taxa downstream were native, though three were nonnative, indicating trade-offs between recolonization and nonnative spread.

Ohio↗

USGS2026

Restoration in motion: Expanded migration and distribution of silver redhorse Moxostoma anisurum and shorthead redhorse M. macrolepidotum

Habitat fragmentation poses a significant threat to migratory species. Dams are a common form of fragmentation, and recent restoration efforts around the Great Lakes have prioritized dam removal. We used acoustic telemetry to describe migratory movements of two redhorse species in the Sandusky and Cuyahoga rivers, Ohio, USA in relationship to habitat reconnection. Shorthead redhorse ( Moxostoma macrolepidotum ) typically migrated from both rivers into Lake Erie between May and July, moving 40–248 km straight-line distance from the river before returning the following spring. We recorded individual cumulative distances up to 809 km between spawning seasons. Shorthead redhorse demonstrated tributary fidelity, but individuals from both rivers co-occurred along southern Lake Erie. Silver redhorse ( M. anisurum ) largely remained in their tagging tributary watersheds year-round. Cuyahoga River silver redhorse moved upstream from March to April 28.8 km on average and passed upstream of the historical Brecksville Dam (removed in 2020), occasionally reaching the next upstream dam. Telemetry data revealed redhorse use of newly available habitat upstream of dam removals and previously undescribed long-range adfluvial migration by shorthead redhorse.

Ohio↗

USGS2026

PFAS exposure suppresses leaf litter decomposition in a stream ecosystem

Decomposition of organic matter is critical to the biogeochemical cycling of carbon and nutrients in all ecosystems. In streams, decomposition can be influenced by anthropogenic impacts, including contaminants. Per- and polyfluoroalkyl substances (PFAS) are resistant to degradation and widespread in freshwater ecosystems, yet little is known about their influence on organic matter processing in streams. We paired an observational field study of a PFAS-impacted stream via food processing wastewater with a 28-day laboratory experiment to investigate PFAS effects on leaf litter processing using multiple lines of evidence. Leaf litter decomposition rates and microbial respiration were significantly lower downstream of the PFAS point-source compared to the paired upstream site. We hypothesized that PFAS suppressed microbial activity thereby resulting in slower decomposition rates. To investigate further and better isolate PFAS, we dosed aquatic mesocosms containing leaf packs with differing concentrations of perfluorooctane sulfonate (PFOS). Over the 28-day incubation, decomposition rates did not differ, but we observed lower respiration rates on day 28 and higher dissolved organic carbon (DOC) in PFOS-dosed mesocosms, suggesting that PFAS may suppress microbial activity and inhibit carbon processing. These results underscore that concerns regarding PFAS contamination extend beyond organismal toxicity to ecosystem-level effects. Further research is needed to understand the scale, implications, and mechanisms responsible for these changes.

Upper Mississippi River Basin↗

USGS2025

Causal networks to inform decisions for ecological restoration

The release of contaminants into the environment can occur from anthropogenic activities, such as oil extraction and transportation, mining, and industrial processes. Remediation associated with reducing contaminant concentrations, and restoration that improves animals and supporting habitat, are often needed to restore ecosystems to their pre-release, baseline condition. We demonstrated the application of Bayesian Decision Networks (BDNs) with two Natural Resource Damage Assessment and Restoration (NRDAR) case studies. We use a stylized case study of riparian restoration following the remediation of a mine-impacted site to evaluate proposed restoration actions aimed at restoring Song Sparrow ( Melospiza melodia ) populations to baseline conditions. We then use a settled NRDAR case with implemented restoration in the Upper Arkansas River (UAR, Colorado, USA) to demonstrate the application of BDNs to evaluate and forecast restoration effectiveness for Brown Trout ( Salmo trutta ) (i.e., restoration effectiveness assessment). The riparian restoration model showed differences in the effects of restoration actions on Song Sparrow populations, with the time to reach baseline generally reduced with increased restoration costs, indicating trade-offs between costs and expected recovery. The UAR model showed recovery of Brown Trout populations (i.e., uplift) in response to improved instream habitat restoration, along with forecasted improvements. While the BDNs we developed were specific to two case studies, the structure is adaptable to a diversity of sites, resources, and actions. We suggest that causal network modeling can provide restoration practitioners with a decision advisory tool useful for a wide range of projects.

Environmental Management↗

USGS2025

Bioaccumulation and transfer of per- and polyfluoroalkyl substances (PFAS) in a stream and riparian food web contaminated by food processing wastewater

We evaluated the bioaccumulation and transfer of per- and polyfluoroalkyl substances (PFAS) in a stream food web contaminated by a food processing facility. Abiotic (i.e., water, sediment, and foam) and biotic (i.e., algae, aquatic insect larvae and adults, fish, and riparian spiders) matrices were sampled upstream and downstream of the facility’s wastewater outfall. Compared with upstream, PFAS concentrations were 600-fold higher in downstream water (mean ∑ 40 PFAS 3.67 ng mL –1 ± 0.48 (standard error)) and reflected inputs from the outfall, with 6:2 fluorotelomer sulfonate (6:2 FTS) dominating the PFAS profile. Within the aquatic food web, perfluorooctanesulfonate (PFOS) was the most biomagnified, and 6:2 FTS was the most biodiluted. In contrast, insect-mediated transfer of PFAS to riparian spiders showed trophic enrichment of 6:2 FTS and dilution of PFOS. We observed significant positive associations between phospholipid membrane-water partition coefficient (log K MW ) and perfluoroalkyl carboxylate (PFCA) chain length on bioaccumulation across most biological matrices, demonstrating that these chemical parameters are predictive of PFAS bioaccumulation potential in the field. Our research reveals important differences in aquatic versus terrestrial exposure for certain PFAS and that biological processes (e.g., trophic interactions and metamorphosis) and chemical properties (e.g., chain length, log K MW , and concentration) control PFAS uptake, bioaccumulation, and transfer in linked freshwater and terrestrial ecosystems.

Environmental Science & Technology↗

USGS2025

Pesticide concentrations in multiple physical and biological stream matrices are impacted by a bioenergy production facility receiving pesticide coated corn seeds

Insecticide and fungicide seed coatings have become prevalent in conventional agriculture in recent decades. From 2015 to 2021, the AltEn bioenergy plant (Mead, Nebraska, USA) generated ethanol from almost 100% unused/expired treated corn seeds. This use of these seeds for ethanol production resulted in the accumulation of large amounts of contaminated wastewater and solid residue, a portion of which was applied to surrounding farmland. To better understand the potential long-term environmental effects from the processing of treated seeds at this facility, five nearby stream sites were sampled in 2022 after the closure of the plant; these included two sites directly impacted by AltEn, one upstream, and two downstream of the impacted sites. Water and sediment were collected in March through July, and algae and fish samples were collected in July for chemical analysis. Overall, 60 pesticide compounds (parents and transformation products) were detected in one or more matrices, including 23 fungicides, 20 insecticides, 16 herbicides, and 1 bacterial growth inhibitor. Pesticide results (maximum detection frequency, maximum concentration) varied substantially by environmental compartment: water (100% multiple pesticides; 4,600 ng L −1 thiamethoxam transformation product (NOA-407475)), algae (100% multiple pesticides, 190 ng g −1 atrazine), sediment (60% dithiopyr, 2.3 ng g −1 acetochlor) and fish (20% pyraclostrobin, 2.2 ng g −1 atrazine). Pesticides associated with treated corn seeds (e.g., insecticides clothianidin, thiamethoxam; fungicides fluoxastrobin, thiabendazole) were detected at significantly higher concentrations ( p < 0.05) in stream water from sites impacted by the AltEn facility compared to non-impacted sites. Study results indicate that pesticides associated with the AltEn facility continue to be a source of contaminants to aquatic systems after the plant’s closure in 2021.

Environmental Toxicology and Chemistry↗

USGS2025

Selenium differentially influences methylmercury retention across mayfly life stages

Though high mercury and selenium concentrations are individually toxic to organisms, there is a hypothesized antagonistic relationship. This potential mercury–selenium interaction is under-studied in aquatic macroinvertebrates, particularly in relation to complex life histories. We examined the proposed effect of selenium on methylmercury accumulation between four life stages for a parthenogenetic mayfly ( Neocloeon triangulifer ). We exposed diatoms to elevated methylmercury concentrations and fed them to mayflies exposed to elevated aqueous selenomethionine. We found some support for the mercury–selenium antagonism hypothesis, but it was context-specific. Selenium reduced methylmercury accumulation in high but not low methylmercury environments. Though terrestrial adult life stages had higher mercury concentrations compared to aquatic larval life stages, cumulative life history transfer factor (LHTF; ratio of methylmercury in adult imago to late instar larvae) differed by treatment. LHTF was constant for all aqueous selenium exposure levels at high dietary methylmercury (selenium impacts on methylmercury uptake and loss) but increased with aqueous selenium exposures at low dietary methylmercury (selenium impacts on methylmercury uptake only), suggesting a synergistic enhancement of MeHg transfer between life stages with increased aqueous Se exposure levels. These results suggest that animals eating adult aquatic insects are exposed to higher concentrations of methylmercury than those feeding on larval insects across selenium and methylmercury levels, but interference of selenium on methylmercury accumulation is only present at high methylmercury environments.

Environmental Science and Technology↗

USGS2025

Accounting for non-random samples with distance sampling to estimate population density

A critical assumption of standard distance sampling is that sampling lines are located such that animals are uniformly distributed as a function of distance from the line. Failure to meet this assumption can introduce bias in the estimator. Many studies have used landscape features, such as roads or rivers, as lines, which can violate assumptions of distance sampling in two ways. First, animals may be attracted or repelled by the landscape feature due to human activity (e.g. along roads) or habitat characteristics associated with the feature (e.g. rivers). Second, sampling along landscape features may not be representative of the larger area of interest. We used auxiliary data to generalize the distance sampling estimator and relax assumptions of a uniform distribution of animals relative to distance from the line (i.e. density gradient) and to allow the distribution of animals to differ by habitat type. The generalized estimator provides unbiased estimates of density within the area sampled but may not be representative of the study area. To address the problem of landscape features providing unrepresentative sampling, we used a resource selection model to estimate the proportion of the population that occurred within the surveyed area to obtain an estimate of abundance for the desired area of inference. We demonstrate our modified distance sampling estimator using white-tailed deer ( Odocoileus virginianus ) in a 972-km 2 study area. We conducted infrared surveys of deer from roads to collect distance-to-transect data. We used locations of radio-collared deer to model the distribution of deer relative to the transects and to develop a resource selection model of deer based on distance to roads, habitat type, elevation and slope to account for roads being a non-representative sample of the study area. Synthesis and applications . When using landscape features as survey lines, the density gradient and deer distribution can introduce either positive or negative bias, which makes it impossible to assess the bias introduced without auxiliary data. The estimator we developed can improve precision because we obtained a better fit to distance observations and accounts for non-random placement of transects with minimal loss of precision.

Journal of Applied Ecology↗

USGS2025

Ecosystem drivers of freshwater mercury bioaccumulation are context-dependent: Insights from continental-scale modeling

Significant variation in mercury (Hg) bioaccumulation is observed across the diversity of freshwater ecosystems in North America. While there is support for the major drivers of Hg bioaccumulation, the relative influence of different external factors can vary widely among waterbodies, which makes predicting Hg risk across large spatial scales particularly challenging. We modeled Hg bioaccumulation by coupling Hg concentrations in more than 21,000 dragonflies collected across the United States from 2008 to 2021 with a suite of chemical (e.g., dissolved organic carbon (DOC), pH, sulfate) and landscape (e.g., soil characteristics, land cover) variables representing external drivers of Hg methylation, transport, and uptake. Model predictions explained 85% of the variation in dragonfly Hg concentrations across the United States. Certain predictor variables were more important than others (e.g., DOC, pH, and percent wetland), and they varied among waterbodies. Variation in Hg bioaccumulation was explained by including habitat and ecosystem type in a hierarchical modeling framework, which confirms the context-dependency of external factors in explaining Hg bioaccumulation across disparate freshwater ecosystems. This continent-scale model provides valuable insights into the processes underlying landscape-scale patterns in Hg exposure risk and demonstrates that drivers of Hg methylation and bioaccumulation are habitat- and ecosystem-dependent.

Environmental Science and Technology↗

USGS2024

Continental-scale nutrient and contaminant delivery by Pacific salmon

The movement of large amounts of nutrients by migrating animals has ecological benefits for recipient food webs1,2 that may be offset by co-transported contaminants3,4. Salmon spawning migrations are archetypal of this process, carrying marine-derived materials to inland ecosystems where they stimulate local productivity but also enhance contaminant exposure5,6,7. Pacific salmon abundance and biomass are higher now than in the last century, reflecting substantial shifts in community structure8 that probably altered nutrient versus contaminant delivery. Here we combined nutrient and contaminant concentrations with 40 years of annual Pacific salmon returns to quantify how changes in community structure influenced marine to freshwater inputs to western North America. Salmon transported tonnes of nutrients and kilograms of contaminants to freshwaters annually. Higher salmon returns (1976–2015) increased salmon-derived nutrient and contaminant inputs by 30% and 20%, respectively. These increases were dominated by pink salmon, which are short-lived, feed lower in marine food webs than other salmon species, and had the highest nutrient-to-contaminant ratios. As a result, the delivery of nutrients increased at a greater rate than the delivery of contaminants, and salmon inputs became more ecologically beneficial over time. Even still, contaminant loadings may represent exposure concerns for some salmon predators. The Pacific salmon example demonstrates how long-term environmental changes interact with nutrient and contaminant movement across large spatial scales and provides a model for exploring similar patterns with other migratory species.

Nature↗

USGS2024

PCB concentrations in riparian spiders (Tetragnathidae) consistently reflect concentrations in water and aquatic macroinvertebrates, but not sediment: Analysis of a seven-year field study

Tetragnathid spiders have been used as sentinels to study the biotransport of contaminants between aquatic and terrestrial environments because a significant proportion of their diet consists of adult aquatic insects. A key knowledge gap in assessing tetragnathid spiders as sentinels is understanding the consistency of the year-to-year relationship between contaminant concentrations in spiders and sediment, water, and macroinvertebrates. We collected five years of data over a seven-year investigation at a PCB contaminated-sediment site to investigate if concentrations in spiders were consistently correlated with concentrations in sediment, water, and aquatic macroinvertebrates. Despite significant year-to-year variability in spider PCB concentrations, they were not correlated with sediment concentrations ( p = 0.186). However, spider PCB concentrations were significantly, positively correlated with PCB concentrations in water ( p < 0.0001, annual r 2 = 0.35–0.84) and macroinvertebrates (p < 0.0001; annual r 2 = 0.59–0.71). Analysis of covariance (ANCOVA) showed that spider PCB concentrations varied consistently with water (β = 0.63) and macroinvertebrate PCB concentrations (β = 1.023) among years. Overall, this study filled a critical knowledge gap in the utilization of tetragnathid spiders as sentinels of aquatic pollution by showing that despite year-to-year changes in PCB concentrations across environmental compartments, consistent relationships existed between spiders and water and aquatic macroinvertebrates.

Science of the Total Environment↗

USGS2023

Mercury isotope values in shoreline spiders reveal transfer of aquatic mercury sources to terrestrial food webs

The transfer of aquatic contaminants, including mercury (Hg), to terrestrial food webs is an often-overlooked exposure pathway to terrestrial animals. While research has implemented the use of shoreline spiders to assess aquatic to terrestrial Hg transfer, it is unclear whether Hg sources, estimated from isotope ratios, can be successfully resolved to inform site assessments and remedy effectiveness. To examine aquatic to terrestrial Hg transfer, we collected shoreline spiders ( Tetragnatha spp.) and aquatic insect larvae (suborder Anisoptera) across a mosaic of aquatic and shoreline habitats in the St. Louis River and Bad River, tributaries to Lake Superior. The fraction of industrial Hg in sediments was reflected in the δ 202 Hg values of aquatic dragonfly larvae and predatory fish, connecting benthic Hg sources to the aquatic food web. Shoreline spiders mirrored these aquatic Hg source signatures with highly positive correlations in δ 202 Hg between tetragnathids and dragonfly larvae ( r 2 = 0.90). Further assessment of different spider taxa (i.e., araneids and pisaurids) revealed that differences in prey consumption and foraging strategies resulted in isotope differences, highlighting the importance of spider taxa selection for Hg monitoring efforts.

Minnesota, Wisconsin↗

USGS2023

A novel approach to assessing natural resource injury with Bayesian networks

Quantifying the effects of environmental stressors on natural resources is problematic because of complex interactions among environmental factors that influence endpoints of interest. This complexity, coupled with data limitations, propagates uncertainty that can make it difficult to causally associate specific environmental stressors with injury endpoints. The Natural Resource Damage Assessment and Restoration (NRDAR) regulations under the Comprehensive Environmental Response, Compensation, and Liability Act and Oil Pollution Act aim to restore natural resources injured by oil spills and hazardous substances released into the environment; exploration of alternative statistical methods to evaluate effects could help address NRDAR legal claims. Bayesian networks (BNs) are statistical tools that can be used to estimate the influence and interrelatedness of abiotic and biotic environmental variables on environmental endpoints of interest. We investigated the application of a BN for injury assessment using a hypothetical case study by simulating data of acid mine drainage (AMD) affecting a fictional stream-dwelling bird species. We compared the BN-generated probability estimates for injury with a more traditional approach using toxicity thresholds for water and sediment chemistry. Bayesian networks offered several distinct advantages over traditional approaches, including formalizing the use of expert knowledge, probabilistic estimates of injury using intermediate direct and indirect effects, and the incorporation of a more nuanced and ecologically relevant representation of effects. Given the potential that BNs have for natural resource injury assessment, more research and field-based application are needed to determine their efficacy in NRDAR. We expect the resulting methods will be of interest to many US federal, state, and tribal programs devoted to the evaluation, mitigation, remediation, and/or restoration of natural resources injured by releases or spills of contaminants

Integrated Environmental Assessment and Management↗

USGS2023

Complex life histories alter patterns of mercury exposure and accumulation in a pond-breeding amphibian

Quantifying how contaminants change across life cycles of species that undergo metamorphosis is critical to assessing organismal risk, particularly for consumers. Pond-breeding amphibians can dominate aquatic animal biomass as larvae and are terrestrial prey as juveniles and adults. Thus, amphibians can be vectors of mercury exposure in both aquatic and terrestrial food webs. However, it is still unclear how mercury concentrations are affected by exogenous (e.g., habitat or diet) vs endogenous factors (e.g., catabolism during hibernation) as amphibians undergo large diet shifts and periods of fasting during ontogeny. We measured total mercury (THg), methylmercury (MeHg), and isotopic compositions (δ 13 C, δ 15 N) in boreal chorus frogs ( Pseudacris maculata ) across five life stages in two Colorado (USA) metapopulations. We found large differences in concentrations and percent MeHg (of THg) among life stages. Frog MeHg concentrations peaked during metamorphosis and hibernation coinciding with the most energetically demanding life cycle stages. Indeed, life history transitions involving periods of fasting coupled with high metabolic demands led to large increases in mercury concentrations. The endogenous processes of metamorphosis and hibernation resulted in MeHg bioamplification, thus decoupling it from the light isotopic proxies of diet and trophic position. These step changes are not often considered in conventional expectations of how MeHg concentrations within organisms are assessed.

Environmental Science & Technology↗

USGS2023

Ecotoxicological studies indicate that sublethal and lethal processes limit insect-mediated contaminant flux

Merolimnic insects can accumulate and transport considerable amounts of aquatic contaminants to terrestrial systems. The rate of contaminant biotransport, termed insect-mediated contaminant flux (IMCF), depends on emergent insect biomass and contaminant accumulation, both functions of environmental concentration. Here we develop a mathematical model of IMCF and apply it to three ecotoxicological studies obtained through the U.S. Environmental Protection Agency's ECOTOX database to determine at which concentration maximum IMCF occurs. Model results demonstrate that the maximum IMCF depends on competing rates of biomass loss and contaminant accumulation and does not necessarily occur at the highest insect or environmental contaminant concentration. Additionally, modeling results suggest that sublethal contaminant effects (e.g., decreased growth) on insect biomass can be an important and potentially underappreciated control on IMCF.

Environmental Toxicology and Chemistry↗

USGS2022

GPS data from 2019 and 2020 campaigns in the Chesapeake Bay region towards quantifying vertical land motions

The Chesapeake Bay is a region along the eastern coast of the United States where sea-level rise is confounded with poorly resolved rates of land subsidence, thus new constraints on vertical land motions (VLM) in the region are warranted. In this paper, we provide a description of two campaign-style Global Positioning System (GPS) datasets, explain the methods used in data collection and validation, and present the experiment designed to quantify a new baseline of VLM in the Chesapeake Bay region of eastern North America. Data from GPS campaigns in 2019 and 2020 are presented as ASCII RINEX2.11 files and logsheets for each observation from the campaigns. Data were quality checked using the open-source program TEQC, resulting in average multipath 1 and 2 values of 0.68 and 0.57, respectively. All data are archived and publicly available for open access at the geodesy facility UNAVCO to abide by Findable, Accessible, Interoperable, Reusable (FAIR) data principles.

Chesapeake Bay area↗

USGS2022

Use of riparian spiders as sentinels of persistent and bioavailable chemical contaminants in aquatic ecosystems: A review

Aquatic ecosystems around the world are contaminated with a wide range of anthropogenic chemicals, including metals and organic pollutants, that originate from point and nonpoint sources. Many of these chemical contaminants have complex environmental cycles, are persistent and bioavailable, can be incorporated into aquatic food webs, and pose a threat to the health of wildlife and humans. Identifying appropriate sentinels that reflect bioavailability is critical to assessing and managing aquatic ecosystems impacted by contaminants. The objective of the present study is to review research on riparian spiders as sentinels of persistent and bioavailable chemical contaminants in aquatic ecosystems. Our review of the literature on riparian spiders as sentinels suggests that significant progress has been made during the last two decades of research. We identified 55 published studies conducted around the world in which riparian spiders (primarily of the families Tetragnathidae, Araneidae, Lycosidae, and Pisauridae) were used as sentinels of chemical contamination of lotic, lentic, and estuarine systems. For several contaminants, such as polychlorinated biphenyls (PCBs), Hg, and Se, it is now clear that riparian spiders are appropriate sentinels. However, many contaminants and factors that could impact chemical concentrations in riparian spiders have not been well characterized. Further study of riparian spiders and their potential role as sentinels is critical because it would allow for development of national-scale programs that utilize riparian spiders as sentinels to monitor chemical contaminants in aquatic ecosystems. A riparian spider sentinel program in the United States would be complementary to existing national sentinel programs, including those for fish and immature dragonflies.

Environmental Toxicology and Chemistry↗

USGS2022

Dietary composition and fatty acid content of giant salmonflies (Pteronarcys californica) in two Rocky Mountain rivers

Many aquatic invertebrates are declining or facing extinction from stressors that compromise physiology, resource consumption, reproduction, and phenology. However, the influence of these common stressors specifically on consumer–resource interactions for aquatic invertebrate consumers is only beginning to be understood. We conducted a field study to investigate Pteronarcys californica (i.e., the “giant salmonfly”), a large-bodied insect that is ecologically and culturally significant to rivers throughout the western United States. We sampled gut contents and polyunsaturated fatty acid composition of salmonflies to compare resource consumption across river (Madison or Gallatin, Montana), sex (male or female), and habitat (rock or woody debris). We found that allochthonous detritus comprised the majority of salmonfly diets in the Gallatin and Madison Rivers, making up 68% of the gut contents on average, followed by amorphous detritus, diatoms, and filamentous algae. Diets showed little variation across river, sex, or length. Minor differences in diets were detected by habitat type, with a higher proportion of diatoms in the diets of salmonflies collected from rocky habitat compared to woody debris. Fatty acid composition generally supported the results of gut content analysis but highlighted the importance of primary producers. The presence of eicosapentaenoic acid (20:5n-3) and alpha linolenic acid (18:3n-3) indicated consumption of diatoms and filamentous green algae, respectively. Our research underscores the importance of a healthy riparian zone that provides allochthonous detritus for invertebrate nutrition as well as the role of algae as an important source of fatty acids.

Montana↗