Assessment of sampling methods to estimate horseshoe crab (Limulus polyphemus L.) egg density in Delaware Bay
[No abstract available]
Geology topics
Publications and source records attributed to D. R. Smith.
[No abstract available]
Concern for the status of horseshoe crab ( Limulus polyphemus ) has increased as harvest for conch and eel bait has increased and spawning habitat has decreased. In early 1999 a workshop was held at the behest of the Atlantic States Marine Fisheries Commission to design a statistically valid survey of horseshoe crab spawning in Delaware Bay. The survey that resulted was a redesign of a volunteer-based spawning survey that began in 1990, and its network of volunteers was relied on to implement the three-stage sampling design in 1999. During May and June of 1999, 163 participants surveyed during the highest of the daily high tides on 16 beaches (8 on each site of Delaware Bay). During the first half of the spawning season, spawning was associated with lunar phases, but moderated by wave height. Disproportionately more spawning occurred within 3 d of the first new and full moons, and spawning activity (measured by an index of female density) was correlated inversely to the percent of beaches with waves ≥0.3 m. Spawning was heaviest on the Delaware shore around the full moon in May in spite of low waves in New Jersey during the new and full moons in May. Number of beaches sampled was the most important factor in determining the precision of the spawning index and power to detect a decline. Explicit consideration of statistical power has been absent from the current debate on horseshoe crab status and harvest. Those who argue against harvest restrictions because of a lack of statistically significant declines take on a burden to show that the surveys they cite have high statistical power. We show the Delaware Bay spawning survey will achieve high statistical power with sufficient sampling intensity and duration. We recommend that future Delaware Bay spawning surveys sample on 3 d around each new and full moon in May and June and increase the number of beaches to ensure high statistical power to detect trends in baywide spawning activity.
Biodiversity surveys are often hampered by the inability to control extraneous sources of variability introduced into comparisons of populations across a heterogenous landscape. If not specifically accounted for a priori, this noise can weaken comparisons between sites, and can make it difficult to draw inferences about specific ecological processes. We developed a terrain-based, paired-site sampling design to analyze differences in aquatic biodiversity between streams draining eastern hemlock (Tsuga canadensis) forests, and those draining mixed hardwood forests in Delaware Water Gap National Recreation Area (USA). The goal of this design was to minimize variance due to terrain influences on stream communities, while representing the range of hemlock dominated stream environments present in the park. We used geographic information systems (GIS) and cluster analysis to define and partition hemlock dominated streams into terrain types based on topographic variables and stream order. We computed similarity of forest stands within terrain types and used this information to pair hemlock-dominated streams with hardwood counterparts prior to sampling. We evaluated the effectiveness of the design through power analysis and found that power to detect differences in aquatic invertebrate taxa richness was highest when sites were paired and terrain type was included as a factor in the analysis. Precision of the estimated difference in mean richness was nearly doubled using the terrain-based, paired site design in comparison to other evaluated designs. Use of this method allowed us to sample stream communities representative of park-wide forest conditions while effectively controlling for landscape variability.
We conducted a comparative study in the Delaware Water Gap National Recreation Area to determine the potential long-term impacts of hemlock forest decline on stream benthic macroinvertebrate assemblages. Hemlock forests throughout eastern North America have been declining because of the hemlock woolly adelgid, an exotic insect pest. We found aquatic invertebrate community structure to be strongly correlated with forest composition. Streams draining hemlock forests supported significantly more total taxa than streams draining mixed hardwood forests, and over 8% of the taxa were strongly associated with hemlock. In addition, invertebrate taxa were more evenly distributed (i.e., higher Simpson's evenness values) in hemlock-drained streams. In contrast, the number of rare species and total densities were significantly lower in streams draining hemlock, suggesting that diversity differences observed between forest types were not related to stochastic factors associated with sampling and that streams draining mixed hardwood forests may be more productive. Analysis of stream habitat data indicated that streams draining hemlock forests had more stable thermal and hydrologic regimes. Our findings suggest that hemlock decline may result in long-term changes in headwater ecosystems leading to reductions in both within-stream (i.e., alpha) and park-wide (i.e., gamma) benthic community diversity.
The United States Endangered Species Act (ESA) requires a biological assessment of any activity that is authorized, funded, or carried out by a federal agency and likely to affect a federally listed endangered species or its critical habitat. We developed a standardized survey protocol for biological assessments of the effects of bridge replacements on 2 federally listed endangered freshwater mussels, Epioblasma torulosa rangiana and Pleurobema clava, found in the Allegheny River, Pennsylvania. The protocol combines qualitative sampling to determine species present with quantitative sampling to estimate density. Data on species present satisfy the minimum requirement of a biological assessment, whereas estimates of density are needed to assess the number of individuals that would die as a result of bridge replacement. Some excavation of substrate is necessary for unbiased population estimates because of species and sex-specific differences in detection at the substrate surface. We reduced the amount of excavation and cost of the survey by using a statistical sampling technique called double sampling, which uses counts from excavating a subset of quadrats to calibrate counts from searching the substrate surface of all quadrats. We applied the survey protocol to the Allegheny River at West Hickory where E. t. rangiana was the 3 rd and P. clava was the 4 th most abundant mussel at the site. Only 31% of P. clava and 52% of E. t. rangiana (80% of females, 45% of males) were detected at the substrate surface. We estimated that 9173 (95% CI: 6309–13,336) E. t. rangiana and 7010 (95% CI: 4462–11,013) P. clava lived within 50 m of the existing bridge and would be affected immediately by bridge construction. (Population estimates did not include mussels too small to be retained on a 6.35-mm-mesh sieve.) Application of the protocol is not limited to biological assessment under the ESA, but is appropriate where site-specific status of freshwater mussel populations is required.
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The ~1.08 Ga anorogenic, A-type Pikes Peak batholith (Front Range, central Colorado) is dominated by coarse-grained, biotite ?? amphibole syenogranites and minor monzogranites, collectively referred to as Pikes Peak granite (PPG). The batholith is also host to numerous small, late-stage plutons that have been subdivided into two groups (e.g. Wobus, 1976. Studies in Colorado Field Geology, Colorado School of Mines Professional Contributions, Colorado): (1) a sodic series (SiO2= ~44-78 wt%; K/Na=0.32-1.36) composed of gabbro, diabase, syenite/quartz syenite and fayalite and sodic amphibole granite; and (2) a potassic series (SiO2= ~ 70-77 wt%; K/Na=0.95-2.05), composed of biotite granite and minor quartz monzonite. Differences in major and trace element and Nd isotopic characteristics for the two series indicate different petrogenetic histories. Potassic granites of the late-stage intrusions appear to represent crustal anatectic melts derived from tonalite sources, based on comparison of their major element compositions with experimental melt products. In addition, Nd isotopic characteristics of the potassic granites [??(Nd)(1.08 Ga) = -0.2 to -2.7] overlap with those for tonalites/granodiorites [ca 1.7 Ga Boulder Creek intrusions; ??(Nd)(1.08 Ga) = -2.4 to -3.6] exposed in the region. Some of the partial melts evolved by fractionation dominated by feldspar. The late-stage potassic granites share geochemical characteristics with most of the PPG, which is also interpreted to have an anatectic origin involving tonalitic crust. The origin of monzogranites associated with the PPG remains unclear, but mixing between granitic and mafic or intermediate magmas is a possibility. Syenites and granites of the sodic series cannot be explained as crustal melts, but are interpreted as fractionation products of mantle-derived mafic magmas with minor crustal input. High temperature and low oxygen fugacity estimates (e.g. Frost et al., 1988. American Mineralogist 73, 727-740) support a basalt fractionation origin, as do ??(Nd) values for sodic granitoids [??(Nd)(1.08 Ga) = +2.2 to -0.7], which are higher than ??(Nd) values for Colorado crust at 1.08 Ga (ca -1.0 to -4.0). Enrichments in incompatible elements (e.g. rare earth elements, Rb, Y) and depletions in compatible elements (e.g. Cr, Sr, Ba) in the sodic granitoids compared to coeval mafic rocks are also consistent with fractionation. Accessory mineral fractionation, release of fluorine-rich volatiles and/or removal of pegmatitic fluids could have modified abundances of Ce, Nb, Zr and Y in some sodic granitoid magmas. Gabbros and mafic dikes associated with the sodic granitoids have ??(Nd)(1.08 Ga) of -3.0 to +3.5, which are lower than depleted mantle at 1.08 Ga, and their trace element characteristics suggest derivation from mantle sources that were previously affected by subduction-related processes. However, it is difficult to characterize the mantle component in these magmas, because assimilation of crust during magma ascent could also result in their observed geochemical features. The Pikes Peak batholith is composed of at least two petrogenetically different granite types, both of which exhibit geochemical characteristics typical of A-type granites. Models proposed for the petrogenesis of the granitoids imply the existence of mafic rocks at depth and addition of juvenile material to the crust in central Colorado at ~ 1.1 Ga.
Virulence of Renibacterium salmoninarum isolates representing five origins was evaluated in eight salmonid hosts; four origins were of Lake Michigan and the fifth was of the Pacific Northwest. The species type strain, ATCC (American Type Culture Collection) 33209, was also included. Each isolate was grown in a kidney disease medium (KDM2) supplemented with 1 % ATCC 33209 culture metabolite; serial 10-fold dilutions were prepared, and groups of fish were challenged by intraperitoneal injection with 0.1 mL of each dilution. A 70-d observation period followed, and bacterial kidney disease (BKD) was diagnosed by the fluorescent antibody technique. Virulence of isolates was quantified as a dose lethal to 50% of fish (LD50) for each host–isolate challenge. In the first set of experiments, 23 isolates were used to challenge groups of brook trout Salvelinus fontinalis. The mean LD50 was 1.087 x 10 6 colony-forming units per milliliter (cfu/mL; SD = 2.022 x 10 6 ), and the LD50 values ranged from 8.457 x 10 6 to 2.227 x 10 4 cfu/mL. Analysis of variance to evaluate the effect of isolate origin on virulence in brook trout revealed no significant difference ( F = 1.502; P = 0.243). Susceptibilities of the other salmonid hosts were evaluated by challenge with six isolates of R. salmoninarum representing each origin and the species type strain. For many of the host–isolate challenge combinations, time to death was highly dependent on the dilution (number of bacteria) injected. In general, the isolates MCO4M, B26, and A34 (all of Lake Michigan origin) tended to be more virulent. Also, LD50 values were dispersed throughout a wider range among the more susceptible hosts. Lake trout Salvelinus namaycush, rainbow trout Oncorhynchus mykiss, and brook trout were relatively resistant to challenge with the strains, whereas coho salmon O. kisutch, domestic Atlantic salmon Saltno salar, and chinook salmon O. tshawytscha were relatively susceptible. Another challenge evaluated the effect of washing R. salmoninarum MCO4M cells before injection into brook trout. The calculated LD50 value, 2.009 x 10 5 cfu/mL, was similar ( X 2 = 0.878; P = 0.645) to that of the unwashed cells (1.163 x 10 5 cfu/mL). Furthermore, times to death for successive dilutions were similar regardless of whether or not the cells were washed.
No abstract available.
The piscivorous nature of the Double-crested Cormorant (Phalacrocorax auritus) is well documented. However, many researchers who have used regurgitated pellets to describe the diet of cormorants report that invertebrates compose a small but consistent portion of the diet. We examined the hypothesis that invertebrates found in pellets are primarily the result of secondary consumption. We used odds ratio analysis to examine associations in 2,846 individual pellets between the presence of specific invertebrate prey and the presence of fish species known to consume those invertebrate taxa. Significant (P < 0.05) relationships occurred between gastropods and pumpkinseed (Lepomis gibbosus) and ictalurids, and between decapods and rock bass (Ambloplites rupestris) and smallmouth bass (Micropterus dolomieu). Significant (P < 0.05) relationships were also found between pelecypods and pumpkinseed and ictalurids. We suggest that the invertebrate prey we observed in pellets were present in the digestive tracts of fish that were consumed by Double-crested Cormorants and hence represent secondary consumption by cormorants. We conclude that consumption of invertebrates by Double-crested Cormorants may be overestimated in the literature in instances where the diet was described using pellets.
Comparisons of bird abundances among years or among habitats assume that the rates at which birds are detected and counted are constant within species. We use point count data collected in forests of the Mid-Atlantic states to estimate detection probabilities for Neotropical migrant bird species as a function of count length. For some species, significant differences existed among years or observers in both the probability of detecting the species and in the rate at which individuals are counted. We demonstrate the consequence that variability in species' detection probabilities can have on estimates of population change, and discuss ways for reducing this source of bias in point count studies.
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