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D. P. Anderson

Publications and source records attributed to D. P. Anderson.

86 records · Page 5Linked to original sources
USGS1979

Plaque-forming cells and humoral antibody in rainbow trout (Salmo gairdneri) induced by immersion in a Yersinia ruckeri O-antigen preparation

Rainbow trout ( Salmo gairdneri ) were exposed to the O-antigen of Yersinia ruckeri by various immunization regimens. The passive hemolytic plaque assay was used to show specific splenic plaque-forming cells (PFC) and passive hemagglutination demonstrated humoral antibody titers in fish injected with or immersed in the antigen preparations. Preceding antigen immersion with a 2-min immersion in either a 5.2 or a 2.6% NaCl solution did not affect the numbers of PFC or levels of antibody. Fish held for 2 min in ethyl m -aminobenzoate methanesulfonate (MS-222 ® ) before antigen immersion showed no significant difference from the control fish. Dosage studies showed that the minimal concentration of antigen for induction of PFC by immersion was 5.0 μg/mL.

Journal of the Fisheries Research Board of Canada↗
USGS1974

Comparison of protection in rainbow trout (Salmo gairdneri) inoculated with and fed Hagerman redmouth bacterins

Rainbow trout ( Salmo gairdneri ) fed 1.0 mg Hagerman redmouth bacterin per fish for 2 wk had no detectable specific, circulating, agglutinating antibody. In fish given a single subcutaneous inoculation of 1.0 mg of bacterin per fish, antibody was present from 3 wk later until 3 mo later, when the final sample was taken. Median lethal doses at various intervals after the bacterins were administered indicated that the inoculated fish could withstand a greater challenge by subcutaneous inoculation of the virulent bacteria than the orally immunized fish. The fish fed the vaccine lost their protection within 6 wk, whereas the inoculated fish had high levels of protection through 3 mo. The degree of protection was also confirmed by a "natural" exposure challenge.

Journal of the Fisheries Research Board of Canada↗