Geology topics

Christopher N. Balakrishnan

Publications and source records attributed to Christopher N. Balakrishnan.

3 recordsLinked to original sources

USGS2018

Population differences in susceptibility to Plasmodium relictum in zebra finches Taeniopygia guttata

Domesticated Australian and Timor zebra finches (Taeniopygia guttata castanotis, and T. guttata guttata, respectively) were inoculated with canary (Serinus canaria) blood containing a Hawaiian isolate of Plasmodium relictum (lineage GRW04), a hemoparasite that causes avian malaria. In two experimental trials, Timor, but not Australian zebra finches developed parasitemia that was detected by microscopic examination of blood smears. In the second trial, in which molecular detection methods were used, a single Australian zebra finch and 5 of 6 challenged Timor birds were positive for the parasite. Additionally, P. relictum DNA was detected in multiple blood samples obtained from Timor birds over the 28 days following challenge. Timor zebra finches may provide a useful, easily maintained, laboratory model for the study of arbovirus/Plasmodium interactions in passerines, but are still inferior to canaries, the traditionally used model of avian malaria infection, in terms of supporting high parasitemia infections.

Avian Diseases↗

USGS2017

Transcriptional response to West Nile virus infection in the zebra finch (Taeniopygia guttata)

West Nile virus (WNV) is a widespread arbovirus that imposes a significant cost to both human and wildlife health. WNV exists in a bird-mosquito transmission cycle in which passerine birds act as the primary reservoir host. As a public health concern, the mammalian immune response to WNV has been studied in detail. Little, however, is known about the avian immune response to WNV. Avian taxa show variable susceptibility to WNV and what drives this variation is unknown. Thus, to study the immune response to WNV in birds, we experimentally infected captive zebra finches ( Taeniopygia guttata ). Zebra finches provide a useful model, as like many natural avian hosts they are moderately susceptible to WNV and thus provide sufficient viremia to infect mosquitoes. We performed RNAseq in spleen tissue during peak viremia to provide an overview of the transcriptional response. In general, we find strong parallels with the mammalian immune response to WNV, including upregulation of five genes in the Rig-I-like receptor signalling pathway, and offer insights into avian-specific responses. Together with complementary immunological assays, we provide a model of the avian immune response to WNV and set the stage for future comparative studies among variably susceptible populations and species.

Royal Society Open Science↗

USGS2017

Susceptibility and antibody response of the laboratory model zebra finch (Taeniopygia guttata) to West Nile Virus

Since the introduction of West Nile virus (WNV) into North America in 1999 a number of passerine bird species have been found to play a role in the amplification of the virus. Arbovirus surveillance, observational studies and experimental studies have implicated passerine birds (songbirds, e.g., crows, American robins, house sparrows, and house finches) as significant reservoirs of WNV in North America, yet we lack a tractable passerine animal model for controlled studies of the virus. The zebra finch ( Taeniopygia guttata ) serves as a model system across a diversity of fields, and here we develop the zebra finch a songbird model for WNV. Like many natural hosts of WNV, we found that zebra finches developed sufficient viremia to serve as a competent host, yet in general resisted mortality from infection. In the Australian zebra finch (AZF) T . g . castanotis , we detected WNV in the majority of sampled tissues by 4 days post injection (dpi). However, WNV was not detected in tissues of sacrificed birds at 14 dpi, shortly after the development of detectable anti-WNV antibodies in the majority of birds indicating successful viral clearance. We compared susceptibility between the two zebra finch subspecies AZF and Timor zebra finch (TZF) T . g . guttata . Compared to AZF, WNV RNA was detected in a larger proportion of challenged TZF and molecular detection of virus in the serum of TZF was significantly higher than in AZF. Given the observed moderate host competence and disease susceptibility, we suggest that zebra finches are appropriate as models for the study of WNV and although underutilized in this respect, may be ideal models for the study of the many diseases carried and transmitted by songbirds.

PLoS ONE↗