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C. J. Deutsch

Publications and source records attributed to C. J. Deutsch.

6 recordsLinked to original sources
USGS2009

Hematology of healthy Florida manatees (Trichechus manatus)

Background: Hematologic analysis is an important tool in evaluating the general health status of free-ranging manatees and in the diagnosis and monitoring of rehabilitating animals. Objectives: The purpose of this study was to evaluate diagnostically important hematologic analytes in healthy manatees (Trichechus manatus) and to assess variations with respect to location (free ranging vs captive), age class (small calves, large calves, subadults, and adults), and gender. Methods: Blood was collected from 55 free-ranging and 63 captive healthy manatees. Most analytes were measured using a CELL-DYN 3500R; automated reticulocytes were measured with an ADVIA 120. Standard manual methods were used for differential leukocyte counts, reticulocyte and Heinz body counts, and plasma protein and fibrinogen concentrations. Results: Rouleaux, slight polychromasia, stomatocytosis, and low numbers of schistocytes and nucleated RBCs (NRBCs) were seen often in stained blood films. Manual reticulocyte counts were higher than automated reticulocyte counts. Heinz bodies were present in erythrocytes of most manatees. Compared with free-ranging manatees, captive animals had slightly lower MCV, MCH, and eosinophil counts and slightly higher heterophil and NRBC counts, and fibrinogen concentration. Total leukocyte, heterophil, and monocyte counts tended to be lower in adults than in younger animals. Small calves tended to have higher reticulocyte counts and NRBC counts than older animals. Conclusions: Hematologic findings were generally similar between captive and free-ranging manatees. Higher manual reticulocyte counts suggest the ADVIA detects only reticulocytes containing large amounts of RNA. Higher reticulocyte and NRBC counts in young calves probably reflect an increased rate of erythropoiesis compared with older animals. ?? 2009 American Society for Veterinary Clinical Pathology.

Veterinary Clinical Pathology↗
USGS2008

Validation of a serum immunoassay to measure progesterone and diagnose pregnancy in the West Indian manatee (Trichechus manatus)

The objective was to validate a high-sensitivity chemiluminescent assay of serum progesterone concentrations for pregnancy diagnosis in manatees. Assay analytical sensitivity was 0.1 ng/mL, with mean intra- and inter-assay coefficients of variation of 9.7 and 9.2%, respectively, and accuracy had a mean adjusted R2 of 0.98. Methods comparison (relative to Siemen's Coat-A-Count RIA) demonstrated r = 0.98, Deming regression slope of 0.95, and an intercept of 0.01. Based on ROC analysis, a progesterone concentration ???0.4 ng/mL was indicative of pregnancy. Assay results were not significantly altered by two freeze-thaw cycles of samples. Characteristic progesterone concentrations during pregnancy were Months 1-4 (1.7-4.7 ng/mL), 5-8 (???1.0 ng/mL), and 10 and 11 (0.3-0.5 ng/mL), whereas two late-pregnant females with impending abortion had progesterone concentrations of 0.1 ng/mL. Among pregnant females, maximum progesterone concentrations occurred in autumn (3.9 ?? 1.8 ng/mL), and were greater during all seasons than concentrations in non-pregnant females (0.1-0.2 ng/mL). Progesterone concentrations were also significantly higher in pregnant females than in non-pregnant females and males. This highly sensitive, specific, and diagnostic assay will be valuable for monitoring pregnancy and abortion in manatees. ?? 2008 Elsevier Inc.

Theriogenology↗
USGS2007

Clinical biochemistry in healthy manatees (Trichechus manatus latirostris)

Florida manatees (Trichechus manatus latirostris) are endangered aquatic mammals living in coastal and riverine waterways of Florida and adjacent states. Serum or plasma biochemical analyses are important tools in evaluating the health of free-ranging and captive manatees. The purpose of this study was to measure diagnostically important analytes in the plasma of healthy manatees and to determine whether there was significant variation with respect to location (free-ranging versus captive), age class (small calves, large calves, subadults, adults), and gender. No significant differences in plasma sodium, potassium, bilirubin, glucose, alanine aminotransferase, or creatine kinase were found among these classes of animals. Compared to free-ranging manatees, captive animals had significantly lower mean concentrations of plasma chloride, phosphate, magnesium, triglycerides, anion gap, and lactate. Captive manatees had significantly higher mean values of total CO2, calcium, urea, creatinine, alkaline phosphatase, gamma-glutamyltransferase, total protein, albumin, and albumin/globulin ratio than did free-ranging animals. Differences in the environments of these two groups, including diet, temperature, salinity, and stress, might account for some of these results. The higher plasma lactate and anion gap concentrations and lower total CO2 concentrations of free-ranging manatees were probably due to greater exertion during capture, but the lack of elevated plasma creatine kinase activity relative to captive animals indicates that there was no serious muscle injury associated with capture. Plasma phosphate decreased and total globulins increased with age. Plasma cholesterol and triglyceride concentrations were highest in small calves. Plasma aspartate aminotransferase was higher in large calves than in adults and subadults, and the albumin/ globulin ratio was higher in subadults than in adults. Plasma total CO2 was higher and chloride was slightly lower in females than in males.

Journal of Zoo and Wildlife Medicine↗
USGS2003

Seasonal movements, migratory behavior, and site fidelity of West Indian manatees along the Atlantic coast of the United States

The West Indian manatee ( Trichechus manatus ) is endangered by human activities throughout its range, including the U.S. Atlantic coast where habitat degradation from coastal development and manatee deaths from watercraft collisions have been particularly severe. We radio-tagged and tracked 78 manatees along the east coast of Florida and Georgia over a 12-year period (1986-1998). Our goals were to characterize the seasonal movements, migratory behavior, and site fidelity of manatees in this region in order to provide information for the development of effective conservation strategies. Most study animals were tracked remotely with the Argos satellite system, which yielded a mean (SD) of 3.7 (1.6) locations per day; all were regularly tracked in the field using conventional radiotelemetry methods. The combined data collection effort yielded >93,000 locations over nearly 32,000 tag-days. The median duration of tracking was 8.3 months per individual, but numerous manatees were tracked over multiple years (max = 6.8 years). Most manatees migrated seasonally over large distances between a northerly warm-season range and a southerly winter range (median one-way distance = 280 km, max = 830 km), but 12% of individuals were resident in a relatively small area (<50 km) year-round. The movements of one adult male spanned >2,300 km of coastline between southeastern Florida and Rhode Island. No study animals journeyed to the Gulf coast of Florida. Regions heavily utilized by tagged manatees included: Fernandina Beach, FL to Brunswick, GA in the warm season; northern Biscayne Bay to Port Everglades, FL in the winter; and central coastal Florida, especially the Banana River and northern Indian River lagoons, in all seasons. Daily travel rate, defined as the distance between successive mean daily locations, averaged 2.5 km (SD = 1.7), but this varied with season, migratory pattern, and sex. Adult males traveled a significantly greater distance per day than did adult females for most of the warm season, which corresponded closely with the principal period of breeding activity, but there was no difference between the sexes in daily travel rate during the winter. The timing of seasonal migrations differed markedly between geographic regions. Most long-distance movements in the southern half of the study area occurred between November and March in response to changing temperatures, whereas most migrations in the northern region took place during the warmer, non-winter months. Manatees left their warm-season range in central Florida in response to cold fronts that dropped water temperatures by an average of 2.0??C over the 24-hr period preceding departure. Water temperature at departure from the warm-season range averaged 19??C, but varied among individuals (16-22??C) and was not related to body size or female reproductive status. The presence of industrial warm-water effluents permitted many manatees to overwinter north of their historic winter range, and for some migrants this delayed autumn migrations and facilitated earlier spring migrations. Southward autumn and northward spring migrations lasted an average of 10 and 15 days at mean rates of 33.5 (SD = 7.6) and 27.3 (SD = 10.5) km/day, respectively. The highest rate of travel during migration was 87 km/day (3.6 km/hr) during winter. Manatees overwintering in southeastern Florida often traveled north during mild weather - sometimes reaching their warm-season range - only to return south again with the next major cold front. Manatees were consistent in their seasonal movement patterns across years and showed strong fidelity, to warm-season and winter ranges. Within a season, individuals usually occupied only 1 or 2 core use areas that encompassed about 90% of daily locations. Most manatees returned faithfully to the same seasonal ranges year after year (median distance between range centers was <5 km between years). Seasonal movements of 4 immature manatees tracked as calves with their mothers

Florida, Georgia↗
USGS1998

Radio-tracking manatees from land and space: tag design, implementation, and lessons learned from long-term study

West Indian manatees (Trichechus manatus) were tracked along the Atlantic coast of Florida and Georgia (N = 83 manatees, n = 439 tag deployments, 1986-1996) and in eastern Puerto Rico (N = 8, n = 43, 1992-1996) using conventional and satellite-based radio-telemetry systems. A floating radio-tag, attached by a flexible tether to a padded belt around the base of the tail, enabled us to track manatees in saltwater environments. The tag incorporated VHF (very high frequency) and ultrasonic transmitters for field tracking and tag recovery, and an Argos satellite-monitored transmitter for remote tracking. We located each animal in the field about twice per week, received more than 60 000 good-quality Argos locations, and recovered tags in over 90% of deployments. The tag was designed to detach from the belt when entangled to prevent injury or drowning, and this often led to premature termination of tracking bouts. We had considerable success, however, in retagging belted manatees without recapture (97% of 392 retagging events). Most individuals were radio-tagged more than once (median = 3.0, maximum = 43) for a median total duration of 7.5 months (maximum = 6.8 yr). Data obtained through Argos have been valuable in addressing questions relating to long-distance movements, site fidelity, and identification of high-use areas. Fine-scale analyses of manatee habitat use and movements may require restricting the data set to the highest location quality or developing new analytical techniques to incorporate locational error. Field tracking provided useful ancillary data on life-history parameters, but sample sizes were small and survival estimates imprecise. Modification of the existing tag design to include Global Positioning System (GPS) functionality, with its finer spatial and temporal resolution, will offer new opportunities to address critical research and management problems facing this endangered species.

Marine Technology Society Journal↗