Use of laboratory spectrometry to predict the detection of phytoplankton luminescence by an airborne Fraunhofer line discriminator
The Fraunhofer line discriminator (FLD), an airborne electro-optical instrument designed to measure solar-stimulated luminescence, has a sensitivity approaching that of current laboratory fluorescence spectrometers. The feasibility of using an airborne FLD for detection of near-coastal and open-ocean phytoplankton was established by using a laboratory fluorescence spectrometer to measure the luminescence of phytoplankton and to determine the minimum concentration detectable with an FLD Laboratory measurements of the excitation spectra of 13 species of phytoplankton (six diatoms, five dinoflagellates and two chrysophytes) were obtained with the emission wavelength held constant at 656.3 nm and the excitation wavelength scanned from 320 to 640 nm. Integrated excitation intensities were normalized to a standard concentration of rhodamine wt dye and the resulting luminescence compared to the minimum detectable FLD level of 0.12 parts per billion (p.p.b.) rhodamine wt. Results demonstrated that all 13 species would be detectable with an FLD at concentrations of 10.0 and 5.0 μg/1 of chlorophyll a and that only one would not be detectable at a chlorophyll a concentration of 1.0 μg/1.